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The Tsimane’ are an indigenous group living in Amazonian Bolivia. Their traditional lifestyle – high pathogen loads, natural fertility, daily subsistence, small communities – arguably reveals some of the same selection pressures experienced throughout most of human evolutionary history; at the same time, many Tsimane’ are transitioning to a more Westernized lifestyle. As such, we study the influence of socio-ecological conditions on social behavior, mental health, and various physical health outcomes assessed by a roaming medical team that also provides health care. We also test adaptive hypotheses and measure the strength and direction of selection on phenotypic traits.